Biomimetic Full-Thickness Skin-on-a-Chip Based on a Fibroblast-Derived Matrix

Current commercially available in vitro skin models do not fully reproduce the structure and function of native human skin, mainly due to their use animal-derived collagen lack a dynamic flow system. In this study, full-thickness skin-on-a-chip (SoC) system that reproduces key aspects vivo cellular microenvironment is presented. This approach combines production fibroblast-derived matrix (FDM) with an inert porous scaffold for long-term, stable cultivation model. The culture dermal compartment under fluid results increased synthesis deposition major FDM proteins, I, fibronectin, compared tissues cultured static conditions. developed SoC includes differentiated epidermal thickness barrier controls. Contrary other platforms include collagen-based matrix, described model presents superior stability physiological relevance. Finally, was quantitatively evaluated via situ transepithelial electrical resistance (TEER) measurements permeation tests. significantly higher TEER lower permeability FITC-dextran. future, innovative low-cost platform could provide new tissue compatible long-term studies study diseases evaluate safety efficacy novel drugs technologies.